Sequencing of the samples was conducted according to the Ion PGM™ 200 Sequencing Kit Protocol (version 6; Ion Torrent). One or more 318 sequencing chips were loaded and run on an Ion Torrent PGM (Ion Torrent) for each sample. Each run was programmed to include 520 nucleotide flows to deliver 200-base reads, on average. Base calling and alignment were performed using the Torrent Suite 3.0 software (Ion Torrent).
