Chunk #76 — Materials and methods — Ion torrent sequencing

Source

Characterizing and measuring bias in sequence data.

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yes

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Template preparation was conducted using the Ion PGM™ 200 Xpress™ Template Kit, following the Ion PGM™ 200 Xpress™ Template Kit protocol (version 3; Ion Torrent). Recovery of the Ion Spheres (ISPs) was done according to the Ion Sphere Particles 200 recovery protocol. Quality of the templated ISPs was assessed using the Guava easyCyte HT8 Cytometer (EMD Millipore, Billerica, MA, USA).