Peritonitis was induced by intraperitoneal (IP) injection with 3 mg MSU crystals (InvivoGen) in 300 µl PBS or PBS alone as a control. After 6 h, the mice were sacrificed and cells from the abdominal cavity were collected with 500 µl PBS lavage. Cells were collected by centrifuging at 300 g for 5 min and subjected to flow cytometry analysis. The supernatant was subjected to ELISA assay. The tissues of the peritoneum were subjected to histochemical staining.
