Impaired glial differentiation-associated gene expression by SCZ hGPCsThe expression of dysregulated genes in SCZ-derived GPCs, as identified by RNA-seq analysis, was assessed by TaqMan Low Density Array (TLDA) RT-qPCR, then compared to that of control hGPCs. Expression data were normalized to GAPDH endogenous control. Mean ddCt values and standard error ranges, calculated from 4 pooled SCZ GPC lines (n = 19) that were individually compared to 3 pooled control GPC lines (n = 10), are shown. The difference in gene expression by SCZ and control hGPCs was assessed by paired t-tests, followed by multiple testing correction by Benjamini-Hochberg (BH) procedure (*** = p <0.01, ** = p < 0.05, * = p <0.1). 48 genes were assessed. 45 genes are shown, excluding the endogenous control and genes that had high proportions of undetermined or unreliable reactions, LRFN1 and NEUROD6. The vast majority of genes were confirmed as dysregulated in SCZ-derived GPCs. Analysis of TLDA data was performed in ExpressionSuite Software version 1.1, supplied by Applied Biosciences.See also Table S3.
