Schizophrenia-derived hGPCs suppress glial differentiation-associated gene expressionRNA sequence analysis reveals differential gene expression by SCZ hGPCs. A, Intersection of lists of differentially expressed genes (DEGs) (log2-fold change >1.00, FDR 5%) obtained by comparison of hGPCs derived from 4 different schizophrenia patients, compared to pooled control hGPCs. B, Network representation of functional annotations for the intersection gene list shown in A. In the upper network, green and red nodes represent down- and up-regulated genes, respectively, and white nodes represent significantly associated annotation terms (FDR-corrected p< 0.01; annotation terms include GO:BP, GO:MF, pathways, and gene families, and nodes are sized by degree). Lower network highlights 4 highly interconnected modules identified by community detection. (C) Top annotation terms identified for each module in B. D, Heat map representation of 12 conserved differentially expressed genes that are associated to module 1 (grey in B, 32.4%), which includes annotations related to neurotransmitter receptor and gated channel activity. E, Heat map representation of 15 conserved differentially expressed genes associated to module 2 (orange in B, 28.7%), which comprises annotations related to cell-to-cell signaling and synaptic transmission. F, Heat map representation of 21 conserved differentially expressed genes associated to module 3 (dark blue in B, 28.7%); annotations related to CNS and glial differentiation. G, Heat map representation of 4 conserved differentially expressed genes that are associated to module 4 (light blue in B, 10.2%), with annotations related to myelination and lipid biosynthesis. The absolute expression in heat maps is shown in UQ-normalized, log2-transformed counts (Li et al., 2015).See also Figures S3 and S4, and Table S2.
