Our anatomically-constrained functional region of interest (ROI) approach used a previously developed anatomical mask for the ROIs to test the proposed hypothesis about the involvement of low versus high LR status regarding insular cortex, medial prefrontal cortex (MPFC), and amygdala based on the Talairach Atlas [53]. The mPFC included primarily the ventral and dorsal anterior cingulate (ACC; including BA 32, 24), spreading into ventromedial PFC regions (BA 10). This medial PFC region was further partitioned into dorsal and ventral extents at z=0, and the insula was partitioned into anterior and posterior portions using a linear interpolation for the location of the middle insular gyrus. Thus, in total there were eight a priori ROIs (bilateral amygdala, dorsal and ventral medial PFC, bilateral anterior and posterior insula). Amygdala regions consisted of 2752 ul (43 voxels) each, anterior insula regions consisted of 9920 ul (155 voxels) each, posterior insula regions consisted of 8512 ul (133 voxels) each, dorsal medial PFC consisted of 21824 ul (341 voxels), ventral medial PFC consisted of 17984 ul (281 voxels), and whole brain consisted of 2,334,464 ul (36476
