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Chunk #13 — Materials and Methods — Analysis — Anatomically-constrained functional regions of Interest

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High versus low level of response to alcohol: evidence of differential reactivity to emotional stimuli.
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ul (155 voxels) each, posterior insula regions consisted of 8512 ul (133 voxels) each, dorsal medial PFC consisted of 21824 ul (341 voxels), ventral medial PFC consisted of 17984 ul (281 voxels), and whole brain consisted of 2,334,464 ul (36476 voxels) (see Figure S1 in the Supplement). A threshold adjustment based on Monte Carlo simulations (conducted via AFNIs in Alpha Sim, a threshold p of .05, two-tailed) was used to guard against identifying false positive areas of activation. The minimum volumes for a significant cluster were: 192 μL for amygdala; 256 μL for insula; 320 μL for medial PFC; and 832 μL for whole brain. Corrected voxel-wise probabilities using Alpha Sim were p: <.0021 for amygdala; <.0015 for anterior insula; <.0016 for posterior insula; <.0008 for ventromedial and for dorsomedial PFC; and <.0001 for whole brain. Center of mass Talairach coordinates (x,y,z) were used and labeled based on visual observation and Talairach Daemon software [53]. The cerebellum was not considered in the analyses. Note that Table S1 in the Supplement presents the results of the whole brain analyses.