Prior exome analyses demonstrated that the observation of even a small number of rare de novo mutations in the same gene among unrelated individuals can provide considerable statistical power to establish association (39). We used the Transmitted And De novo Association (TADA) test as a statistical method for risk gene discovery based on gene-level recurrence of de novo and inherited mutations within the classes of variants that we found enriched in OCD (29, 40). Parameter calculations and a detailed description of the method are given in Supplemental Methods.
