Chunk #9 — Materials and methods — Solutions for oral self-administration

Source

Permanent impairment of birth and survival of cortical and hippocampal proliferating cells following excessive drinking during alcohol dependence.

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Alcohol (10% w/v) was prepared with 95% ethyl alcohol and tap water. Glucose (3%) and/or saccharin (0-0.125%; Sigma, St. Louis, MO) was added to the water or alcohol solutions to achieve the appropriate concentration. Sweetened alcohol was only used in the early stages of training and was faded out so that animals were lever pressing for 10% w/v alcohol (unsweetened) versus water within the first few weeks of training.