Production of GABAergic interneurons from human embryonic stem cells (hESCs) makes therapeutic transplant possible, and multiple protocols to create MGE-like cells from hESCs exist (Southwell et al., 2014). The MGE produces cortical and striatal interneurons as well as oligodendrocytes, but the proportion of specific cell types generated in vitro is not known because these studies have characterized gene expression at the population level (Kessaris et al., 2006). It is also not clear how human interneuron identity and development in culture differs from primary tissue.
