A Seurat object was created from the data from each pool from the HT assay using the gene expression count matrix from the Cell Ranger output (17 objects total). Cells with below 800 or above 11,250 genes, above 125,000 molecules, or above 10% mitochondrial RNA were removed from further analysis. These are commonly used quality control metrics to remove low-quality cells or multiplets. Each pool was then normalized using the scTransform() function in Seurat.
