A Signac object, containing both RNA and ATAC-seq data, was created for each pool from the multiome assay from the hdf5 file from the Cell Ranger output (23 objects total). Cells with below 800 or above 20,000 genes, below 800 or above 500,000 detected RNA molecules, or above 20% mitochondrial RNA were removed from further analysis. An additional round of filtering was performed using the ATAC-seq data. The following cells were removed from further analysis:
