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Chunk #10 — MATERIALS, METHODS AND SAMPLES — Marker Selection and Genotyping

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Replication of association between schizophrenia and ZNF804A in the Irish Case-Control Study of Schizophrenia sample.
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Following discussion with the Cardiff group, we genotyped 3 of the most promising SNPs from the report of genomewide association in the Cardiff sample, rs1344706 in ZNF804A, rs6490121 in NOS1 and rs9922369 in RGRIP1L. Based on the results in these markers, we then completed genotyping of 11 additional LD-tagging SNPs (tSNPs) in ZNF804A. We first manually included rs1344706 (associated in the original study) in the test alleles set and then selected additional tSNPs using the TAGGER39 algorithm with default criteria of r2≥0.8 and minor allele frequency (MAF)≥0.2 as implemented in Haploview 4.040 using HapMap data release 22/Phase II, Apr 07. Including rs1344706, we genotyped a total of 12 SNPs in ZNF804A. Markers are shown in Table 1; orientation and alleles are reported on the genomic (+) strand (rs1344706 is reported here as A/C, not T/G as in the original report23).