All markers were genotyped with Taqman Assays-on-Demand (Applied Biosystems, Foster City, CA). To ensure uniformity and accuracy, all reaction steps were performed using the Eppendorf 5075 automated liquid handling platform. Genotypes were called using an automated allele scoring platform41. For quality control, we exclude any individual with >50% missing genotypes. We analyzed duplicate genotype data for 35 duplicate pairs (11 cases and 24 controls) and use discordant genotypes in these duplicates to estimate genotyping error rates.
