Upstream regulator analysis identified putative proteins and chemicals that may be responsible for the pattern of changes in expression (Supplementary Tables S3 & S4, respectively). This analysis indicated increased activity for TP53, NFκB, INSIG1, cAMP and ATF4, genes that regulate large numbers of other genes, as well as dexamethasone, which indicates changes similar to cortisol treatment. Regulators that appear to be less active include HIF1A, RARA, insulin receptor, estrogen receptor and SREBF1/2. SREBF1/2 regulate the expression of genes in the cholesterol pathway.
