In the sequencing stage, the coding exons and their flanking intronic regions of the 17 genes were sequenced in 320 AA cases with co-occurring diagnoses of AD, CD and OD and 380 AA controls, and 440 EA cases with co-occurring diagnoses of AD, CD and OD and 380 EA controls (Table 1). Each DNA pool contained DNAs from 20 subjects of the same sex, population, and phenotypes (AD, CD and OD or none of these). The total length of the target regions was 34,544 bp. One hundred percent of the target regions were sequenced at > 1000× coverage, and the mean coverage of all of the bases in the target regions per pool was 41,869× (about 1,000× per individual chromosome; Figure S1) indicating very high depth.
