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Chunk #12 — MATERIALS AND METHODS — Blood Alcohol Concentrations

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Postnatal binge-like alcohol exposure decreases dendritic complexity while increasing the density of mature spines in mPFC Layer II/III pyramidal neurons.
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Blood samples were collected from a tail clip of each AE pup 90 minutes after the second alcohol intubation on PD 4 in order to determine the blood alcohol concentration (BAC). BACs were assayed from the plasma of each blood sample using an Analox GL5 Alcohol Analyzer (Analox Instruments, Boston, MA).