At PD 4, litters were randomly assigned to one of three conditions: SC, SI or AE. SC animals were weighed daily but otherwise left undisturbed. The SC condition received no intubation treatment, as opposed to both the SI and the AE animals. Intragastric intubations occurred from PD4-9 for both the SI and AE groups. SI animals were intubated, on the same schedule as the AE animals, and the tube was removed after 10–15 seconds without the infusion of any solution. AE animals were intubated and given a daily dose of 5.25g/kg of alcohol, which was divided into two intubations each day, two hours apart. A third intubation of milk (no ethanol) was administered two hours after the second alcohol dose. Solely on PD 4, a fourth intubation of milk solution was given four hours after the second alcohol dose in order to compensate for reduced milk intake. All pups were weaned on PD 23 and subsequently housed in social conditions of 3–4 rats of the same sex per cage.
