Chunk #40 — Methods — DNA amplification

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Combined analysis of circulating β-endorphin with gene polymorphisms in OPRM1, CACNAD2 and ABCB1 reveals correlation with pain, opioid sensitivity and opioid-related side effects.

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Amplification of ABCB1 was conducted with one PCR at 94°C, 2 min, 94°C 30 sec, 60°C 30 sec, 68°C 1 min 30 cycles, 4°C hold using the AccuPrime Taq DNA polymerase (Invitrogen, Carlsbad, CA). Primers used were forward 5’-GAGCCCATCCTGTTTGACTG-3’ and reverse 5’-ACTATAGGCCAGAGAGGCTGC-3´.