Chunk #41 — Methods — DNA amplification

Source

Combined analysis of circulating β-endorphin with gene polymorphisms in OPRM1, CACNAD2 and ABCB1 reveals correlation with pain, opioid sensitivity and opioid-related side effects.

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The PCR product was purified with the PCR Clean-Up Kit (Invitrogen, Carlsbad, CA) using the Magtration 12GC system and the concentration were determined using NanoDrop (Long beach, CA, USA).