the periphery of the neurospheres. Nearly all of the NSCs of the undifferentiated neurospheres contained 5-MeC (Figure 1K). The core of the differentiating neurospheres exhibited a mosaic of high and weak levels of 5-MeC marks (Figure 1L). The periphery and migrated zones generally exhibited little 5-MeC-im, but moderate levels of 5-MeC-im were occasionally seen scattered throughout these zones. The expression of DNMT3a in the undifferentiated neurospheres was variable; the core and periphery of differentiating neurospheres was similar to undifferentiated neurospheres, while the migrated NSCs down-regulated DNMT3a.
