The undifferentiated neurospheres contained high expression of DNMT1, while the expression in the core of the differentiating neurospheres was a mosaic of highly-expressing and weakly-expressing NSCs that were more intensely stained (p-value < 0.05), compared to the undifferentiated (Figure 1G). In cells beginning differentiation at the periphery of the neurosphere, a notable ring (edge of neurosphere) of relatively strong DNMT1 expression (Figure 1H) was observed (p-value < 0.05), compared to the undifferentiated. Migrated NSCs, however, significantly down-regulated DNMT1 (p-value < 0.05), compared to the periphery. Interestingly, the expression of MBD1 was very low or absent in NSCs of the undifferentiated neurospheres (Figure 1I). The NSCs at the core and periphery of the differentiating neurospheres expressed MBD1 at moderate levels, differing from the undifferentiated (Figure 1I vs 1J). Migrated NSCs down-regulated MBD1 significantly (p-value < 0.05) compared to the cells at the periphery of the neurospheres. Nearly all of the NSCs of the undifferentiated neurospheres contained 5-MeC (Figure 1K). The core of the differentiating neurospheres exhibited a mosaic of high and weak levels of 5-MeC marks (Figure 1L). The periphery and
