Chunk #27 — Methods — Next generation sequencing — Sequencing

Source

The long non-coding RNA NEAT1 is responsive to neuronal activity and is associated with hyperexcitability states.

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For total RNA, sequencing was performed using the Illumina HiSeq2500 platform with 100 bp paired-end sequencing with a fragment size of approximately 292 bp. Illumina TruSeq version 3 chemistry was used for cluster generation and sequencing.