Chunk #26 — Methods — Next generation sequencing — Total RNA library preparation

Source

The long non-coding RNA NEAT1 is responsive to neuronal activity and is associated with hyperexcitability states.

Embedded

yes

Text

500 ng of total RNA was used as input material for library preparation using the TruSeq Stranded Total RNA Sample Prep Kit (LT) (Illumina, CA, USA) according to manufacturer’s instructions. Individual libraries were indexed as recommended by Illumina.