Prior to library preparation, each total RNA sample was DNase-treated using TURBO DNase (Life Technologies, CA, USA) according to the manufacturer’s instructions followed by extraction, ethanol precipitation and resuspension in nuclease-free H2O. DNase-treated RNA quality was assessed on an Agilent Technologies 2100 Bioanalyzer with an RNA 6000 Nano kit (Agilent Technologies, CA, USA) according to the manufacturer’s instructions. RNA concentration was measured using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, MA, USA).
