paperKB
coga / coga-kb
Help
Sign in

Chunk #13 — Methods — RNA expression analysis

Source
FKBP5 variation is associated with the acute and chronic effects of nicotine.
Embedded
yes

Text

Fwd AAGTTTGCAGAGCAGGATGC, FKBP5 Rev GGCCCTCAGGTTTCTCTTCT. The relative level of gene expression was determined with the ΔΔCt method using the mean value for two experiments. Consistent with array-based results, the mean cycle threshold was associated with cortisol levels at time point 1 (Figure 1) in a regression model adjusted for age, sex and race (p<0.05, Figure S1).