The correlation between DNA methylation at the Mecp2 REs and expression of Mecp2 isoforms was determined using the Pearson’s correlation analysis and linear regression. The Pearson’s correlation coefficient (r) was calculated for average methylation against each Mecp2 isoform, over entire regions and for individual CpG sites within each region. The strength of correlation was considered as follows: weak, 0 < r <0.3; moderate, 0.3 < r <0.4; strong, 0.4 < r <0.7; or very strong, 0.7 < r <1.0. A negative r-value indicates an inverse/negative correlation whereas a positive r-value indicates direct/positive correlation. Statistical significance was determined at P <0.05.
