Genomic DNA was isolated as described in the previous section. Primer design and bisulfite pyrosequencing experiments were conducted as a service by The Hospital for Sick Children (SickKids), Toronto, Canada, as reported elsewhere [53], using the primers listed in Table 5. The regions analyzed for the methylation patterns are shown in Figure 1B. Specific CpG dinucleotides that are analyzed within each region are shown in Additional file 1.
