10 min, 45 cycles of 94°C for 0.5 min, 62°C for 0.5 min, 72°C for 0.75 min, followed by 72°C for 10 min. The PCR products were denatured with Hi-Di Formamide (Applied Biosystems, Foster City, CA) at 95° for 5 min. The fragments were electrophoresed using a DNA Sequencer (ABI 3100, Applied Biosystems) with a fluorescent ladder ROX350 (Applied Biosystems) as an internal size marker and analyzed with GENOTYPER software (Version 3.7).
