SNP Genotyping With Taqman Assays. SNPs rs2851060, rs713455, and rs3730251 were genotyped using Allelic Discrimination software (SDS v2.1) and Taqman assays for ABI Prism 7900HT Sequence Detection System (Applied Biosystems). To distinguish between each SNP allele, one Taqman minor groove binding (MGB) probe was labeled with VIC reporter dye and another labeled with FAM reporter dye included with SNP sequence-specific forward and reverse primers. Reactions were performed in a 384-well format in a total reaction volume of 5 μl containing 6.0 ng of genomic DNA and 1× TaqMan Universal PCR Master Mix without AmpErase UNG (Applied Biosystems) using 50°C for 2 min, 95°C for 10 min, and 40 cycles of 95°C for 0.25 min, 60°C for 1 min. Fluorescent signals between 500—660 nm were analyzed for each plate and genotypes were scored using automated software.
