Most assays were designed for the Sequenom MassArray system (Sequenom, San Diego, CA) using MassArray Assay Design Software. The assays were done using the hME or iPLEX assay format (Sequenom, San Diego, CA); in both cases, alleles were discriminated by mass spectrometry. Assays were tested on two groups of 40 unrelated individuals from the Coriell European-American and African-American samples. SNPs that were not in Hardy-Weinberg equilibrium in both populations were not genotyped in the sample. Some SNPs were genotyped using the Illumina technology on a BeadLab station with GoldenGate chemistry, as described previously (Dick et al., 2008).
