Ethanol self-administration was monitored in adult (8 weeks) HS-CC mice using the two-bottle choice preference test (10% ethanol vs. water) (see Metten et al. 2014). Mice were individually housed and given 1 week of habituation, with continuous access to food and tap water, accessible from two 25-ml graduated glass cylinders fitted with rubber stoppers and stainless steel sipper tubes. Mice were acclimated to the taste and effects of ethanol by progressively increasing the concentration of ethanol (0%, 5% and 10% v/v) every 4 days. Every other day, the position of the ethanol bottle was reversed to control for side preferences.
