Dopamine concentration after acute heroin injection was measured from AcbC of SHRs and WKYs using in vivo microdialysis and high-performance liquid chromatography. Gene expression was measured from Acb of SHRs and WKYs using RT-qPCR. Epigenetic modifications near Crem were assessed in Acb of SHRs and WKYs using either chromatin immunoprecipitation (ChIP) and qPCR, or bisulfite conversion and sequencing. Primary cortical and striatal neurons derived from Long-Evans embryos were co-cultured for dendritic neuromorphology. For details, see Supplementary Information.
