Clusters CBPC, HYPEP3 and SCINH2 showed dual presence of GABA (inhibitory) and glutamate (excitatory) in the same cluster, but manual inspection indicated that this was artifactual (for example, contamination of Purkinje cells by granule cells in the cerebellum). Clusters OBINH4, SZNBL and DEINH6 showed no neurotransmitter by trinarization, but manual inspection indicated GABA. Clusters where ‘neurotransmitter genes’ were not expressed homogenously were also identified. All these manual interventions were documented in the “Comment” field of Table S3, in each loom file and in the wiki.