To investigate global behaviors in adults following AE binge treatment (P28-37), we assessed young adult mice (P63-P72; Figure 1) for locomotor open field activity, Morris Water Maze acquisition and reversal learning, as well as prepulse inhibition and anxiety-like behavior in the elevated plus maze. In the water maze, mice from both groups easily learned with a visual cue on the platform (Figure S2A, <10 seconds). Similarly, in the hidden platform task, both groups learned in parallel over four days of testing, as indicated by reduced time to find the hidden platform (Figure S2B). Results from a probe trial also supported equivalent learning ability in both groups, since both spent significantly more of their search time in the correct quadrant, and less than 20% in the incorrect quadrants, with no difference between the treatment groups (Figure S2C). Both groups of mice received 3 days of reversal learning training with the platform in the opposite quadrant, with both groups showing a modest reversal (Figure S2D). The reversal learning probe trial indicated significant differences between young adult control and young adults following adolescent binge ethanol treatment (Figure 5).
