Following reversal training, the control mice no longer demonstrated a strong preference for the original platform location in quadrant 1, spending roughly an equal amount of time also searching quadrant 3, where the platform had been re-located during reversal. In contrast, the ethanol-treated mice significantly spent more than twice as much time in quadrant 1 (35.6%) than in the quadrant with the new platform location (15.5%), demonstrating a perseverative response pattern (Figure 5A; **p<0.01). Further, the ethanol-treated group spent significantly less time than the control mice searching quadrant 3, where the platform had been re-located during reversal († p<0.05, compared to controls). These findings demonstrate adolescent binge ethanol treatment of C57BL/6 mice does not cause young adult spatial learning deficits, but disrupts reversal learning.
