We tested a spectrum of other behaviors to determine if adolescent binge ethanol caused other differences in adult mice. Behaviors associated with sensorimotor gating, locomotor activity, and anxiety showed no significant differences between groups. Prepulse inhibition is a measure of sensorimotor gating. Adult mice that received alcohol during adolescence showed nonsignificant reductions in prepulse inhibition across all the prepulse decibels tested (Figure S3, 2-way ANOVA: p=0.11, F=2.931). Reductions at two of the prepulse decibels measured (78 and 90dB) reached statistical significance using a t-test (*p<0.05). Locomotor activity in the open field was the same in both groups across a one-hour period (Figure S3B, 2-way ANOVA: p =0.27, F=1.30). In the elevated plus maze, a measure of anxiety-like behavior, both groups spent similar amounts of time in the open arms (Figure S3C, control: 35.9 sec ± 8.3, ethanol 39.4 sec ± 4.5, p=0.73, t test) and the closed arms (control: 203.6 ± 16.1, ethanol 210.7 ± 7.1, p=0.72, t test). Center time in the open field, another measure of anxiety-like behavior and activity in a novel environment, was also unchanged between
