Chunk #27 — 4. Selectively bred high alcohol-consuming rat lines and their phenotypic characteristics — 4.3. High alcohol-drinking and low alcohol-drinking rats
The high alcohol-drinking, HAD, and low alcohol-drinking, LAD, lines of rats were developed using a within-family selection and rotational breeding design (which decreases the level of inbreeding) from N/NIH heterogeneous stock rats at Indiana University School of Medicine in Indianapolis, Indiana, USA (Li et al., 1993). The N/NIH line of rats was derived from eight inbred strains (ACI, BN, BUF, F344, M520, MR, WKY and WN) at the National Institutes of Health (Hansen and Spuhler, 1984). Two separate colonies were used to breed HAD and LAD lines of rats, such that replicate (HAD1 vs. LAD1 and HAD2 vs. LAD2) lines are available to assess genotypic and phenotypic differences. As a caveat, the selective breeding program of the HAD2-LAD2 line-pair has trailed that of the HAD1-LAD1 line-pair by approximately 1 year (i.e., the HAD2-LAD2 line-pair has trailed the HAD1-LAD1 line-pair by approximately 2 generations of selection). A genotypic or phenotypic trait that is present in both replicate lines would convey greater confidence in the importance of that trait for the development of high or low alcohol drinking behavior than if the
