Chunk #15 — Materials and Methods — Genetic Data and Statistical Analyses

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Fine mapping of calcineurin (PPP3CA) gene reveals novel alternative splicing patterns, association of 5'UTR trinucleotide repeat with addiction vulnerability, and differential isoform expression in Alzheimer's disease.

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GOLD software (http://www.sph.umich.edu/csg/abecasis/GOLD) allowed graphical display of LD between SNP and microsatellite markers (Abecasis and Cookson, 2000). PHASE version 2.1 (http://www.stat.washington.edu/stephens/software.html) provided haplotype estimates (Stephens and Donnelly, 2003; Stephens, Smith, & Donnelly, 2001). Chi-square tests (Microsoft Excel, MS Office 2003) identified genotype or allele frequency association for PPP3CA microsatellite and SNP markers. Statistical analyses for PPP3CA brain region expression differences used Prism 3.0 software (GraphPad, Inc., San Diego, CA).