Female C57BL/6 mice obtained from Jackson Labs were acclimated to the animal facility (22 °C) for one week on a reverse light/dark cycle (lights on at 2000 hours, off at 0800) in group-housing conditions (4 per cage) with ad libitum access to food and water. After acclimation, females were separated into individual housing for one week before initiation of the drinking paradigm. Prenatal exposure of mice to 10% w/v alcohol was performed using our previously published drinking in the dark method (Brady et al., 2012). Briefly, females were provided either a solution of 10% w/v alcohol sweetened with 0.066% (w/v) saccharin or a 0.066% (w/v) saccharin-only solution for 4 h starting 2 h into the dark cycle (1000–1400 hours). The concentration of alcohol was increased to 10% over the initial 4 days of the paradigm. After one week of drinking at the 10% w/v alcohol level, a single female was introduced to a singly housed male immediately after the drinking period; the female was removed from the male cage the following morning at the beginning of the dark cycle at
