at the 10% w/v alcohol level, a single female was introduced to a singly housed male immediately after the drinking period; the female was removed from the male cage the following morning at the beginning of the dark cycle at 0800 hours. Food and water were available ad libitum during the breeding period. Females were bred for 2 days and consumed alcohol or saccharin solutions during the same 4-h period each day. One week after the last day of breeding, pregnancy was determined by monitoring weight gain. As the cell culturing method consisted of embryonic tissue (E15–E17), date of conception was not determined. Pregnant dams were sacrificed 13–15 days after the last day of mating to obtain appropriately aged tissue. Alcohol consumption was monitored before and after breeding and throughout pregnancy for several breeding rounds of mice used in these studies. Alcohol intake during pregnancy averaged 7.33 ± 0.35 g/kg body weight per day. This is similar to our previously published data (7.17 ± 0.17 g/kg body weight per day), which resulted in blood alcohol levels of 88.3 ± 11.5 mg/dL 4 h after drinking 10% w/v alcohol solution (Brady et al., 2012). This is approximately equal to 19.3 mM
