At D10 (24 hrs after transfection), cultured neurons were treated for 48 hrs with control solution or ApoE2, ApoE3, or ApoE4 (10 µg/ml). Cell lysates were collected at D12 in passive lysis buffer (Promega), and dual luciferase reporter assays were performed according to manufacturer instructions using a plate-reading luminometer with reagent auto-injectors Apollo LB915 (Berthold Technologies). Samples were compared by subtracting the background activity of the reference control, and then normalizing the firefly luciferase activity of each sample to its Renilla luciferase activity.
