This was similar to the one-bottle DID test described above by Rhodes et al. (2005) except that two bottles (instead of one) containing either 20% ethanol or water were used (Blednov and Harris, 2008). The ethanol and water bottles remained in place for 3 hr. After their removal, mice had unlimited access to one bottle of water. Bottle positions during 3 hr access were changed daily to avoid potential side preferences. The ethanol and water bottles were weighed before placing and after removal from experimental cages.
