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Chunk #13 — MATERIALS AND METHODS — Participant Selection for Association Studies

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Association study of 182 candidate genes in anorexia nervosa.
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above and each of these participants was related to one individual in the primary sample. Genomic DNA samples from these individuals were sent to Illumina for genotyping. Of these individuals, we removed 11 participants whose DNA samples failed genotyping; 220 participants who were included for quality control purposes; and 264 participants included to support pilot projects (27 males with AN, 121 first-degree relatives, and 116 individuals with eating disorder diagnoses other than AN). No participants were excluded due to excessive genotype missingness (all were ≤0.021). Thus the overall sample includes 1,762 female participants (1,085 cases and 677 controls). In this analysis sample, there were 10 families with two second-degree relatives, 15 families with two third-degree relatives, and three families with two fourth-degree relatives.