decreased to 75 microliters per reaction, and the PCR reaction volume increased to 100 microliters. The product was PCR amplified for 16 cycles with primers mws13 and mws20, and purified with 1.2 volumes of Ampure XP beads. The purified DNA was combined with 2.5 micrograms Cot-I DNA and 500 picomoles each of oligonucleotides mws60 and mws61, and a second round of capture21 was performed with 1.5 picomoles of pooled biotinylated oligonucleotides. A final PCR reaction was carried out for 8–10 cycles with primers mws13 and mws21, which contains a fixed index sequence for multiplexing. After cleanup with 1.2 volumes of Ampure XP beads, the product was sequenced on an Illumina HiSeq 2500.
