Chunk #61 — Experimental Procedures — HEK cell transfection

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Posttranscriptional regulation of BK channel splice variant stability by miR-9 underlies neuroadaptation to alcohol.

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In miRNA experiments, BK 3’UTR+/− plasmids (0.0442 fmol/well) were transfected alone, or with one of two miRNA precursors: miR-9 and miR-135b (200 pmol/well). BK mRNA was isolated for quantitation 24 hours after transfection.