Chunk #62 — Experimental Procedures — HEK cell transfection

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Posttranscriptional regulation of BK channel splice variant stability by miR-9 underlies neuroadaptation to alcohol.

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For electrophysiological analysis cells were transfected with a BK variant (BKALCOREX, BK-STREX or BK-Insertless in pVAX) together with the expression plasmid (πH3-CD8) for the α subunit of the human CD8 lymphocyte surface antigen (GeneBank M12824), allowing for identification of transfected cells with CD-8 antibody-coated beads (Dynal/Invitrogen) as previously.