FACS analysis of fixed cells (adapted from [46]). (a) FACS analysis of MGE cells generated from H9 cells after Nkx2.1 staining at day 21 of differentiation. (b–d) Combined and temporal treatment with IWP-2, SAG and FGF8 resulted in robust induction of MGE cells from H9 and H7 hESCs as well as iPSCs, assayed after 25 days of differentiation. The image in the insets is Hoechst-labeled nuclei of the same microscope field. Scale bar: 100 µm.
