Gene expression analysis during MGE derivation of H9 cells (adapted from [46]), assayed by real-time PCR (Mean ± SEM; n = 3) on days 0, 3, 7, 14 and 21 of differentiation. The expression level of Nanog, Oct4, Nkx2.1, Gsx2, Dlx2 and Lhx6 was analyzed at each time point after differentiation. The expression level of each gene was normalized to that of GAPDH.
