Human MGE cells generated GABAergic interneurons (adapted from [46] and [29]). (a–l) Immunocytochemistry and cell counting analysis after 6 weeks of differentiation (Mean ± SEM; n = 4, P < 0.05, two tailed t-test), showing the expression of β-tubulin, GABA, Lhx6, GAD, Sox6, Calbindin (Calb), Parvalbumin (PV) and Somatostatin (SST). Immunostaining was performed on H9-derived cells except a and c, in which H7-derived cells were analyzed. The image in the insets is Hoechst-labeled nuclei of the same microscope field. (m–s) Immunohistochemical analysis of transplanted H7-derived MGE cells 4 months after transplantation, showing the expression of SST, PV, Calretinin (Calr), Neuropeptide Y (NPY), Calb among transplanted human nuclei (hNuc)+ cells. Scale bar: 100 µm.
